Fiber Photometry System

Dual Color Multichannel Fiber Photometry System

Specifications

Features & Benefits

System Overview - Model R810

Detecting activity changes of neurons in the central nervous system in real-time, the fiber photometry system sums up the overall fluorescence of neurons to express overall activity without reflecting the activity of individual neurons. RWD Fiber Photometry System utilizes the 410nm wavelength as the reference channel while the 470nm light source excites GCaMP. The significance of the reference channel lies in increasing self-control, reducing spontaneous fluorescence, eliminating signal artifacts caused by animal’s intense exercise and fiber winding, expressing two kinds of proteins at the same site, detecting levels of calcium change in different groups of neurons, and true recording and analysis of dual-color fluorescence signals.

System Overview - Model R811

R811 dual color multichannel fiber photometry system is compatible with optoelectronics equipment. It has different wavelengths of excited light, 410nm and 470nm, of which 410 is used to acquire reference signal and eliminate noise. The system can record signal of green fluorescence indicator like GCaMP and dLight or neurotransmitter probe.
And also it can be applied to record the neural signal for 9 sites at the same time.

Features & Benefits

LED excitation light sources

Two kinds of LED excitation light sources at 410 & 470 nm, of which 410 is the reference channel, allows for a more accurate fluorescence signal acquisition.

Software

The software has four pre-set fluorescence mode options, which can be applied to different experimental application scenarios.

Channels

It can support up to 9 channels, suitable for high-throughput calcium signal acquisition or simultaneous recording of multiple neural nucleus groups

Signals

High-performance and low noise CMOS camera can collect calcium fluorescence signals of multiple channels at up to 300 frames per second (fps).

Inputs

When the external event is marked, the time automatically inputs to achieve a synchronous recording with behavioral video, and the external stimulus time synchronization accuracy is up to 10ms.

Input ports

4 input ports support a variety of external signal input and automatic marking while 4 output ports support TTL signals to trigger external equipment.

Technical Specifications (R810 & R811)

Model R810
Frame Rate of CMOS	0~300 FPS
sCMOS camera resolution	2048 * 2048 px
Exposure time setting range	1~100 ms
Fluorescence band	500-550 nm
Central wavelength of excitation light	410 nm, 470 nm
LED power adjustable percentage	0~100%
410nm LED Output Power Range	0~100 μW
470nm LED Output Power Range	0~100 μW

Model R811
Wavelength of excitation light	410nm 470nm
Power	Min 0 µW, Max ≥ 100 µW
Number of channels	9
Frame rate of fluorescent sampling	Max 250fps
Digital signal interface	4 Input and 4 Output
Signal output	Output frequency 0-500Hz
Marking	Manual marking (10), automatic marking (4), ROI marking (9)
Behavior camera	19201080(30fps) 1280720(60fps) . Switchable among multiple frame rates of resolution
Compatible	Compatible with optogenetics for recording and stimulation at the same site