FUSION Absolute
The First Imaging System with Absolute Protein Quantification
System Overview
Designed to exceed your expectations, the FUSION ABSOLUTE is the first ultra-precise photon counting instrument enabling you to achieve absolute quantification. Its automated analysis capabilities allow you to detect, decode, and quantify your protein of interest with certainty. This high-end system, dedicated to all your chemiluminescence and fluorescence applications, combines unrivalled optical sensitivity and optimum visualisation of your results to offer you the greatest level of performance. With its wide choice of customizable light sources, it’s a one-of-a-kind molecular imager opening the way to infinite multispectral imaging possibilities. Discover a world of extreme precision and technical innovations where your experiments know no limits.
Enjoy faster and deeper analysis. Our system detects the weakest signals and decodes your images by turning data into information while maintaining its integrity. The calculations are already done for you. The visualization of your results combines the calculations made by the system and the image taken so that you can easily communicate your results. With its multispectral imaging and spectral unmixing technologies, KUANT Spectral unmixing makes your life easier and solves your crosstalk and autofluorescence issues. Get more accurate detection and measurement in your multi-labeled samples thanks to our powerful N.M.F. (non-negative matrix factorization) algorithm.
Features & Benefits
Reagents & Fluorophores
Fluorophores: Alexa 488 - Cy 2 - Alexa 532 - Alexa 555 - Alexa 680 - Alexa 700 - Alexa 750 - Cy 7- Alexa 790
Intercalating agents:
Ethidium Bromide, SYBR Safe, Midori Green, Gel red, SYBR Green.
All You Need in One System
Genomics: DNA analysis (mutation, methylation etc...), RNA (gene expression...) Proteomics: Analysis of proteins molecular weight (analysis of mutation, expression, etc.)
Western blot:
Biomedical research, molecular biology research, cancer research etc.
Absolute Quantification
Absolute quantification provides a precise value for sample quantities, expressed in absolute units (radiance in photons/s/cm2/sr) to allow researchers to compare and integrate data across studies.
Absolute Flexibility
The non-display version of the FUSION ABSOLUTE offers similar performance in a compact format. With versatile application capabilities, imaging western blots, DNA gels and RNA, and in vivo luciferase.
Powerful Multispectral Imaging Capabilities
Capture images at multiple wavelengths to extract comprehensive information from a sample.
Absolute Calibration
Absolute NIST Traceable Calibration Imaging is based on traceable reference values and ensures your system delivers accurate and reproducible data.
Absolute Quality
Powerful and robust all-in–one standalone system made of aluminium and stainless steel. Its design has been optimized by our R&D team to offer you the widest touchscreen in the market while perfectly fitting in your lab. Adjust screen orientation and view vibrant images in high resolution.
Multiplexing
Detect several different proteins in a single sample. This technique will save you time and money, while giving you a complete analysis of the target proteins.
Absolute Time Saver
The camera is designed to adjust automatically in the Z-axis based on your sample’s position and size. No more tray levels required.
The Best Optics
The f/0.70 aperture of our lens provides faster imaging and better sensitivity compared to all other imagers in the market.
Impressive Dynamic Range
FUSION ABSOLUTE custom-made f/0.70 lens combines sensitivity and optical performance for very faint light conditions (picogram level). The system achieves the best signal-to-noise ratio and can easily detect large intensity difference between bright and faint bands before reaching saturation. The broad linear dynamic range enables quantification of target proteins with absolute confidence.
Models & Specifications
FX6
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Chemiluminescence and Fluorescence
Can be upgraded anytime to cover multiple imaging applications (i.e. standard gel documentation)
FX7
-
Chemiluminescence and Fluorescence
Can be upgraded anytime to cover multiple imaging applications (i.e. standard gel documentation)
Camera & Optics
| FX6 | FX7 |
|---|---|
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Unrivalled custom-made lens f/0.70 Scientific grade 16-bit CCD camera Grade 0, zero defect |
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Image resolution: 20 megapixels Native resolution: 2838×2224 -55° C maximum cooling differential from ambient & -30°C absolute and regulated cooling via three stages Peltier thermoelectric cooler |
Image resolution: 10 megapixels Native resolution: 2160×2160 -90° C maximum cooling differential from ambient & -65°C absolute and regulated cooling via a double cooling system & four stages Peltier |
Software
| All Fusion Models |
|---|
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License free software for image acquisition with full GLP compliance |
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Molecular weight and distance calculation |
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Absolute quantification – Colony counting |
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3D Dynamic Scan – CFR21 Part 11 ready |
Hardware Capabilities
| All Fusion Models |
|---|
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Intelligent darkroom concept |
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Integrated touchscreen or non-display models available for FX6 and FX7 |
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Motorized optical lens |
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Z-axis motorized camera (automatic zooming and recognition of the sample position) |
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Choice of 9 Absolute Spectra Capsules (excitation light sources) from 365 to 780nm |
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Motorized 10 positions filter wheel |
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Software control of the lighting |
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Automatic visible lighting adjustment |
Accessories & Add-ons
| Accessories/Add-ons |
|---|
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• Spectra Capsules: 9 LED capsules available (365nm to 780nm)
• Interchangeable Pads: Black Pad, White Light Pad (VIS), Super Bright LED Pad (UV-A+UV-B), Blue Light LED Pad, Green Light LED Pad • Advanced Bio-1D Software for quantification |
Applications
Chemiluminescent Western, Northern, and Southern Blot
DNA & RNA gels and fluorescence stain imaging with UV-Pad or Blue-Pad
Fluorescence Western Blot
Colorimetric stained protein gels, X-Ray film, autorads, SSCP gels, colony dish and flask imaging with Sky-Light-Pad or UV-Pad + conversion screen
Customize Your FX Spectra Model
UV: 365 nm
Blue: 440 nm
Blue: 480 nm
Green: 530 nm
Orange: 580 nm
Red: 640 nm
NIR: 680 nm
NIR: 740 nm
IR: 780 nm
Past Webinars - Fusion
Publications & Articles
FUSION ABSOLUTE: The First Imaging System with Absolute Protein Quantification
London, ON — Scintica is proud to introduce the FUSION ABSOLUTE, the world’s
Application Highlight: Studying Coronary Vessel Dynamics
Application Highlight: Studying Coronary Vessel Dynamics The coronary vessel wall plays a central
Publication Highlight: Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia
Publication Highlight: Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia