Frequently Asked Questions: MycoFog Biodecontamination System
This Frequently Asked Questions document was created to help answer some of the most asked questions by people newer to MycoFog.
1. What is MycoFogâ„¢?
MycoFogâ„¢ is an advanced biodecontamination system that uses hydrogen peroxide (Hâ‚‚Oâ‚‚) fogging to eliminate microbial contamination from incubators, workstations, and other enclosed spaces.
2. What reagents can I use with MycoFogâ„¢?
MycoFogTM reagent, 4 bottles + 4 wicks. For incubators <200L | MFR-1Bx-K |
MycoFogTM reagent, 4 bottles + 4 wicks. For incubators >200L and <350L | MFR-2Bx-K |
MycoFogTM reagent, 2 bottles + 2 wicks. For incubators >350L and 500L | MFR-3Bx-K |
MycoFogTM reagent, 2 bottles + 2 wicks. For incubators >500L. Use 2 MycoFog instruments if ineterior volume is >1000L | MFR-4Bx-K |
- MycoFogâ„¢ Hâ‚‚Oâ‚‚ reagent (our approved reagent for optimal performance):-
- Other compatible Hâ‚‚Oâ‚‚ solutions can be used (but please ensure proper concentration and purity). Please note that MycoFogTM has not validated other H2O2 formulations for use with the MycoFogTM instrument, so at this time we do not have supporting data except for the MycoFogTM reagent.
3. How often should I perform a MycoFogâ„¢ decontamination cycle?
For optimal contamination control, we recommend performing a MycoFogâ„¢ cycle once per month per incubator.
 4. How do I prepare MycoFogâ„¢ for a decontamination cycle?Â
- Ensure the incubator is empty (remove cultures, labware, and water pans).
- Set the temperature to 45°C (or use the 37°C protocol for 4 hours).
- Disable gas control (COâ‚‚ and humidity settings).
- Place MycoFogâ„¢ on the at mid-level inside the incubator for optimal fog distribution.
- Press the function button to start the cycle.
 5. How long does the MycoFog cycle take?
- Standard Cycle: 180 minutes at 45°C
- 37°C Protocol: 4 hours for <200L chambers, 5 hours for larger chambers
 6. Can I use MycoFog™ in large incubators?
Yes! MycoFog reagents come in four different pack sizes, each reagent formulation is designed for a size of Incubator/Glovebox/Workstation:
MycoFogTM reagent, 4 bottles + 4 wicks. For incubators <200L | MFR-1Bx-K |
MycoFogTM reagent, 4 bottles + 4 wicks. For incubators >200L and <350L | MFR-2Bx-K |
MycoFogTM reagent, 2 bottles + 2 wicks. For incubators >350L and 500L | MFR-3Bx-K |
MycoFogTM reagent, 2 bottles + 2 wicks. For incubators >500L. Use 2 MycoFog instruments if ineterior volume is >1000L | MFR-4Bx-K |
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 7. What is the recommended relative humidity before starting a cycle?
- MycoFog™ works best when the incubator’s starting relative humidity is ambient.
- The device achieves proper humidity during the cycle.
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 8. Can I use MycoFog™ in an incubator that contains powered electronics?
Yes MycoFog™ has been tested with electronic devices such as the Etaluma Lumascope and the Sartorius Incucyte which perform normally after repeated decontamination cycles.
 9. My MycoFog™ instrument is not producing enough spray. What should I do?
- Check the Wick: Remove wick ensure it is fully saturated and reseat wick. Ensure wick holder is seated properly.
- Check the Reagent: Ensure the correct amount is added and that only MycoFogâ„¢ Hâ‚‚Oâ‚‚ reagent or an approved formulation is used.
- Clean the Fogger: Use a cotton swab with vinegar to clean the nebulizer element.
- Charge the Battery: Low power can reduce spray intensity—fully charge before use.
 10. My MycoFog™ stopped mid-cycle. How do I fix it?
- Battery may be low —recharge the unit.
- Restart the device by turning it off and on again
 11. The reagent is leaking—what should I check?
- Ensure the reagent reservoir is properly sealed.
- Confirm that the instrument is level and not tilted.
- Do not overfill— use only the recommended reagent volume.
 12. How long does the battery last
- A fully charged battery will perform approximately 10 cycles.
- We recommend fully charging the instrument before each use if repeated uses are done on different days
 13. Can MycoFog™ operate while charging?
Yes, MycoFogâ„¢ can function while connected to a power source.
 14. How do I clean my MycoFog™ instrument?
- After each use, rinse the reagent reservoir with distilled water.
- If fogging volume decreases, clean the nebulizer disk with vinegar and a cotton swab. Â
- Use a fresh wick for each cycle for consistent performance.
 15. What quality control checks are performed before shipping?
Each MycoFogâ„¢ instrument is tested to ensure:
- Proper flow rate within the acceptable range
- Residual reagent volume is below the maximum allowable level
 16. Can MycoFog™ be used in a GMP environment?
Yes! Our solvent-resistant instrument labels allow for alcohol wipe-downs before GMP suite entry. We also offer solvent-resistant reagent labels on request.
 17. How do I validate MycoFog™’s effectiveness?
- Use one of the MycoFogTM PiTY Kits (Prove it to Yourself Kits), which include Biological Indicators (BI’s) to confirm a 6-log reduction in microbial contamination.
Prove it to Yourself Kit (MycoFogâ„¢ Instrument + 2 bottles MycoFogâ„¢ reagent + 4 Biological indicators + 2 wicks). For incubators <200L | MF-PitY-1Bx |
Prove it to Yourself Kit (MycoFogâ„¢ Instrument + 2 bottles MycoFogâ„¢ reagent + 4 Biological indicators + 2 wicks). For incubators >200L and <350L | MF-PitY-2Bx |
Prove it to Yourself Kit (MycoFogâ„¢ Instrument + 2 bottles MycoFogâ„¢ reagent + 4 Biological indicators + 2 wicks). For incubators >350L and <500L | MF-PitY-3Bx |
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 18. Need Help?
For troubleshooting, orders, or technical support, contact us at: support@mycofog.com
