Overview:

Western blotting remains a cornerstone technique in life science research. However, inconsistent signals, high background, and limited dynamic range can compromise data quality and slow progress. In this webinar, we’ll break down how to optimize your western blot workflow using Vilber’s FUSION Absolute, from sample preparation through detection, with a practical focus on imaging strategies that deliver reliable, publication-ready results.

The session will begin with essential reminders on best practices and common pitfalls in western blot preparation, followed by a deep dive into chemiluminescence detection. You’ll learn how to troubleshoot common imager-related challenges, such as weak signals, saturated bands, non-uniform membranes, and excessive background.

We’ll then explore epi-fluorescence detection as a powerful alternative, highlighting its stable signal over time, wide dynamic range, and multiplexing capabilities. Attendees will gain a clear understanding of how fluorescence differs from chemiluminescence and how to resolve imaging-specific issues like channel crosstalk and background noise.

The webinar concludes with practical guidance on choosing the right imaging system to match your research needs, showing how modern multimodal imagers, such as the FUSION Absolute, enable flexibility, sensitivity, and confidence across both chemiluminescent and fluorescent western blot applications.

Key Learning Objectives:

1. Improve western blot reliability and reproducibility: Identify critical workflow steps and avoid common preparation and imaging mistakes that lead to weak, saturated, or inconsistent results.
2. Understand when to use chemiluminescence vs. epi-fluorescence: Compare detection principles, strengths, and limitations, including signal stability, dynamic range, and multiplexing, to select the best approach for your experiments.
3. Optimize imaging performance with the right imager: Learn how advanced multimodal imaging systems like the Fusion Absolute help overcome common detection challenges and support high-quality western blot data across applications.