The Prospect T1 has been specifically designed for preclinical imaging of small animals including mice and rats. These species require very high-resolution images (up to 30µm) to observe anatomical structures, and considerations to monitor changes in hemodynamics due to their high heart rates. This tablet based, high frequency, compact ultrasound system is a cost-effective alternative to other preclinical ultrasound systems on the market, allowing individual labs to harness the power of this real-time imaging modality.
The system is well equipped with standard ultrasound capabilities including B-mode, EKV mode, M-mode, Color and Power Doppler, Pulsed Wave Doppler, Tissue Doppler, and Contrast imaging (reference subtracted and harmonic options are available). In all modes, the RAW digital RF data can be saved for further analysis should this be desirable. Additionally, the system’s capabilities may be expanded to acquire 3D B-mode images, perform image guided injection, or to perform shear wave elastography.
The standard configuration and capabilities of the Prospect T1 allow for researchers with a wide variety of research interests to utilize the system; these applications include cardiovascular research, cancer biology, general abdominal/anatomical imaging, developmental biology, as well as ophthalmology applications.
The suite of analytical tools and measurement packages allow imagers to quickly generate quantitative results over the course of their longitudinal studies.Included with the system is the scanning platform which may be equipped with either a heated mouse or rat sized platform, which has integrated physiological monitoring. This animal handling apparatus has been ergonomically designed to allow for easy positioning of the ultrasound probe to acquire the desired images, while being easy to clean as needed between imaging subjects.
Currently there are three different single element probes available, the choice of which to use is dependent on the animal to be imaged and the anatomical targets, center frequencies range from 20-50MHz, with broadband frequencies up to 60MHz.
Offline data analysis is also available to ensure that the system time is reserved for image acquisition, while data can be transferred to an analysis station to perform measurements as needed. Images may be exported in standard formats, while data may be compiled into reports and exported into .csv files for compilation and statistical analysis.
Features & Benefits
The Prospect T1 is a cost effective high-frequency preclinical ultrasound system for mice, rats and other small animals, optimized to study the hemodynamics and vascular function at the laboratory benchtop instead of from inside a core imaging facility.
Standard Imaging Modes
- Pulsed Wave, Tissue, Color, Power Doppler
- Contrast Mode – linear and non-linear/harmonic
The standard system can be used by a wide variety of researchers, studying various disease models including cardiovascular disease, cancer research, developmental biology, etc. Movement of the excitation source over the entire FOV ensures consistent and reproducible results over the course of a longitudinal study.
System may be configured with up to 3 different probes:
System has 2 available animal platforms:
- Mouse table
- Rat table
Available optional probes and animal platforms allow the systems to be configured to meet a specific research team’s need.
Additional probes and platforms may be added in the future to expand capabilities as needed.
ECG Gated Kilohertz Visualization (EKV) mode available on all systems
This mode allows users to acquire B-mode images of the heart with up to 400fps; these images provide exquisite detail of cardiac motion and may be used with any of the standard measurement tools.
RAW data, and corresponding import scripts are available in all modes.
Researchers who would like to look at the digital RF data from any mode can easily access the data off the system and import into third party software for further analysis.
System includes a tablet-based acquisition computer with compact scanning platform
Small system footprint which requires less space than conventional systems.
The system may be easily moved and does not need to reside in a core facility.
The system can fit within a biosafety cabinet if needed, to help researchers who may be working with immunocompromised animals, or those with other concerns.
Simplified image guided injection mount
With the probe and injection mount connected, once the needle and imaging plane are aligned, the user can move the probe/needle as needed to perform the injection.
Comprehensive measurement package is included on the system, with available optional offline analysis software license
Users can quickly perform the measurements they desire, compile data reports, and generate quantifiable outputs from images and spectrograms.
This analysis may be done on the system, or at an offline analysis workstation.
Add-on hardware/software options are available:
- 3D Motor including software for volume measurements
- Image Guided Injection Mount, with software image guide
- Shear Wave Elastography/ Acoustic Radiation Force push probe and software integration
These optional components are available to those researchers who have specific needs.
They are not included in all systems to keep costs as low as possible but can be easily added to any system when needed.
System has been designed with an intuitive workflow with touch screen user interface (keyboard and mouse also come with the system)
Preclinical researchers can quickly start their studies, acquire and store images from the various modes available, and quickly review their data and perform their required measurements.
Non-linear (first harmonic) contrast agent imaging is available using both the 20MHz and 40MHz probes
This allows for increased sensitivity to microbubble contrast agents, without the need for reference subtraction that is required for linear contrast agent imaging.
This improves signal sensitivity, and risk of motion artefact within the images.
Mouse Left Ventricle in Long Axis – B-Mode
Mouse Carotid Artery – B-Mode
Mouse E15 Embryo – B-Mode
Mouse Mammary Fat Pad Tumor – 3D B-Mode
ECG gated kilohertz visualization (EKV)
Mouse Left Ventricle in Long Axis – B-Mode with 30fps
ECG gated kilohertz visualization (EKV)
Mouse Left Ventricle in Long Axis – EKV with 120fps, now possible up to 400fps
Mouse Left Ventricle in Short Axis – M-Mode
Mouse Aortic Arch – Color Doppler
Mouse Subcutaneous Tumor – Power Doppler
Pulsed Wave (PW) Doppler
Mouse Mitral Valve Inflow – PW Doppler
Mouse Mitral Valve Annulus – Tissue Doppler
Mouse subcutaneous tumor – Non-linear contrast agent imaging with regions of interest drawn
Mouse subcutaneous tumor – Non-linear contrast agent imaging Time vs. Intensity plots for drawn regions of interest
Shear Eave Elastography
Shear wave elastography is a technique used to study and quantify the mechanical and elastic properties of various tissues including liver, breast, tendons, muscles, etc. An acoustic radiation force is generated by a secondary, non-imaging, element mounted on the side of the imaging probe. The software is used to program the pulse sequence to generate the shear wave; the wave will propagate faster through stiffer tissues, as well as along the axis of various musculoskeletal structures. The analysis software generates a colored elastogram which is overlaid upon a B-mode image. Tissues with focal or diffuse lesions may be identified using this technique.
Propagation of Shear Wave through mouse liver
Shear Wave Elastogram showing focal lesion in mouse liver
Models & Specifications
PB506e – Center Frequency 50MHz (30-60MHz)
PB406e – Center Frequency 40MHz (20-50MHz)
PB207e – Center Frequency 20MHz (10-30MHz)
Mouse Platform 17.2 x 14.2cm
Rat Platform 28.3 x 18.2cm
Standard - Advanced & Optional
Pulsed Wave / Tissue / Color / Power Doppler
Contrast Mode (Linear and Non-Linear/Harmonic
RAW data available for all modes
Accesories & add-ons
3D Motor and Acquisition/Analysis Software
The 3D motor connects to the imaging platform to precisely move the animal bed with imaging subject under the probe. The acquisition software allows the user to select the distance the motor will travel and the step size. The acquired imaging slices are then reconstructed into a 3D image. The visualization and analysis software allows the user to move through the acquired image, view it as a 3D surface, and make volume measurements.
Mammary Fat Pad Tumor in Mouse – 3D Image
Mammary Fat Pad Tumor in Mouse – 3D Volume = 263mm3
Mouse Hind Limb Muscle – 3D Image
Mouse Hind Limb Muscle – 3D Volume = 97.6mm3
Prospect T1 Imaging Gallery
The Prospect T1 is routinely used for cardiovascular imaging of a variety of small animal models, including mice and rats.
Real-time images are provided of the heart and a wide variety of blood vessels.
The system can be used to assess both systolic and diastolic function in the heart; as well to image and assess flow through any number of blood vessels, including the aorta, pulmonary, carotid, renal, splenic, and femoral arteries etc. Veins, including the vena cava and portal veins may also be assessed.
Imaging may be performed in utero, as early as day 1 after delivery, all the way through adulthood. Cardiovascular function may be of interest as disease progresses, perhaps induced by surgical intervention, transgenic mutation, or systemic treatment with a therapy or toxic compound. Ultrasound imaging is non-invasive, so the same measurements may be taken repeatedly over the course of a longitudinal study.
Mouse Left Ventricle in Long Axis in B-Mode
Mouse Left Ventricle in Short Axis in M-Mode with Measurements to Calculate Systolic Function
Mouse Mitral Valve Inflow in PW Doppler with Measurements to Calculate Diastolic Function
Mouse Mitral Annulus in Tissue Doppler with Measurements to Assess Diastolic Function
Caption – Mouse Aortic Arch in Color Doppler
Mouse Carotid Artery in B-Mode
Mouse Pulmonary Artery in PW Doppler to Assess RV Outflow
Publications & Articles
Read more about how our high-frequency ultrasound system has leverage the research work of many scientists around the globe.
Allogeneic Expanded Human Peripheral NK Cells Control Prostate Cancer Growth in a Preclinical Mouse Model of Castration-Resistant Prostate Cancer
Fangming Wang, Xuejiao Dong, Jing Wang, Feiya Yang, Donghua Liu, Jianlin Ma, Shuai
(April 5, 2023) Webinar: Prodigy Open-Platform Research Ultrasound System Overview Overview:In this webinar,
Ursolic acid prevents doxorubicin‐induced cardiac toxicity in mice through eNOS activation and inhibition
Synergistic inhibitory effects on hepatocellular carcinoma with recombinant human adenovirus Aspp2 and oxaliplatin
Mouse Abdominal Imaging Guide: Imaging the Liver, Hepatic Vessels, Portal Veins and Gallbladder
How the Prospect T1 high-frequency ultrasound system can be used in preclinical developmental