In Vivo Endomicroscopy

In Vivo Imaging on a Pathway
to Pathology in Real Time

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Features & Benefits

System Overview

The FIVE2 (ViewnVivo) B30 is the latest miniaturised fluorescence endomicroscope from Optiscan, optimized for real-time preclinical research in vivo imaging in animal models; and while the FIVE2 (ViewnVivo) B30 comes to life in vivo, there is no reason why it can not be used for ex vivo research as well. Combining all that is required to get you started, the FIVE2 (ViewnVivo) B30 will have you capturing stunning and unparalleled images immediately.

The main components are:

  • Confocal Processor
  • Optiscan Imager (software that controls the Confocal Processor and captures images)
  • Miniaturised Probe
  • Client PC, Monitor, Keyboard and Mouse
  • Animal Handling Stage and Probe Positioner
  • 3D Visualization and Analysis Software

Move to the “Key Features” and “Components” tab to learn more.

Optiscan - ViewnVivo - Point Scanning vs Bundle Fibre

Point Scanning Confocal Technology
Point Scanning Confocal is a patented technology designed and developed by Optiscan over many years and is responsible for the stunning full field of view image capture delivered by FIVE2 (ViewnVivo).

Embedded within the tip of the miniaturized probe is a complex mechanical scanner, comprising a pair of lenses that focus the laser at the exact depth required. The laser is delivered, and the returning light is captured through a single optical fibre that is moved across the field of view (scanning) to capture the entire image. The system controls the speed at which the optical fibre scans, and can be adjusted accordingly along with the power of the laser, to provide optimal results.

The following image is a comparison of legacy Bundle Fibre technology (left) and Optiscan Point Scanning Technology (right). Image set A presents a sample scan at full screen resolution. Image set B represents a focal area (point of interest) of the sample image, and Image set C is the zoomed view of this focal area. As you can see, legacy Bundle Fibre technology does not offer a level of clarity and detail any where close to Point Scanning Technology.


Features & Benefits

Superior Optics – Stunning Image Quality

Optiscan’s patented imaging technology and variably adjustable imaging depth feature permit researchers to capture a level of image detail that is unmatched from any other single miniaturised confocal microscope probe. Capture high-resolution real-time in vivo images with submicron detail and view them in stunning full-screen quality for faster Preclinical Research insights.



Miniaturised no longer means compromise. Not only does the FIVE2 (ViewnVivo) platform capture images comparable to those previously only possible from larger and more expensive fixed/bench mounted microscopes, giving you the flexibility to view cellular and sub-cellular structures in vivo that can quickly take your research to another level.

Optical Sectioning

Through interactive, continuously variable depth control (z-axis), capture images as optical sections to generate 3D visualisations.

Image: Visceral adipose tissue stained with LYVE-1, AF488 and Hoechst for nuclei – Courtesy of Enyuan Cao, Monash Institue of Pharmaceutical Sciences

Real-time In Vivo Insight

As someone who values the benefit of detailed insight, you will appreciate the ability to unlock the ultimate and most direct observations of systems biology in vivo. No longer do you need to try to observe living tissue in an unnatural manner. With FIVE2 (ViewnVivo) not only can you see it in its natural state but you can enable longitudinal studies and keyhole procedures whilst reducing terminal procedures.


Don’t let inflexible bench mounted microscopy be a limiting factor. The flexibility of a miniaturised probe enables investigation not possible through fixed benchtop microscopes. FIVE2 (ViewnVivo) lets you take your imaging to your animal model and position it at any angle of approach to allow you to interrogate the structures of interest, in vivo.

No Probe Change

There is no need to change probes within a procedure, although you can change the probe form factor to suit the investigation, without restarting the system. Unlike other technologies, with FIVE2 (ViewnVivo) you can dynamically adjust imaging parameters using a single probe enabling uninterrupted imaging.

Never Miss an Image

Capture images and their associated meta-data on-demand even if your hands are full, or automatically as a series of image depths (z-stack). Images can even be captured retrospectively from the live image stream through the ‘roll-back’ functionality.

No Dedicated Technician or Facility Required

FIVE2 (ViewnVivo) delivers maximum flexibility in a platform so simple and small that it doesn’t require a dedicated technician or facility, meaning it can be used by virtually anyone and still capture unbelievable images that will validate your investigations. The self-calibrating system and probe lets the user turn it on and use it.


Arthroscopy and Cartilage

Front of Eye Imaging

Infectious disease

Tumour Molecular Expression and Angiogenesis

Molecular and Receptor Targeted Imaging (Cancer)

Tissue Micro-Flora


B30 Specification Summary

Imaging Resolution
< 0.5 μm lateral; <4.5 μm axial
Imaging Depth
0 – 400μm (without moving or changing the probe) Note: effective imaging depth is dependent on specimen/tissue being interrogated
3μm (precision 1μm)
Field of View
475μm x 475μm at full resolution plus zoom
Aspect Ratios
1:1, 16:9, 16:10, 4:3, 5:4 Note: scanning speed changes depending on the selected aspect ratio
488nm (Blue)
Laser Power
10μW – 1000μW (1mW) – adjustable in 10μW increments
Probe Outer Diameter
Standard Probe
66mm (straight rigid) – Others available
Probe Cable Length
Filter Wheel
12 Position (8 Standard filters included) *4 custom filters can be added
Image Capture
Single Frames, Z-Stacks and [retrospective] Roll-back
Image Output
Captured as TIFF and Multi-page TIFF (including embedded meta-data)
Maximum Scan Speed
6 fps

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