(June 17, 2020) WEBINAR: Fluorescence in vivo Endomicroscopy: Imaging and Analysis (Co-Hosted w/Invicro)
Advanced benchtop microscopes can deliver resolution on the order of nanometers. However, they are highly invasive and require hours to days of tedious sample preparation, increasing the possibility of artifacts. Imagine a microscope that can capture images in real time directly from a living animal!
Befitting this, laser confocal endomicroscopy (LCE), with its probe-based imaging approach, delivers cellular resolution, while being minimally invasive. Many scientists and imaging core facility managers refer to it as “virtual histology”; it captures real time microscale images like classical histology, in a non-destructive manner. It is a cutting-edge imaging modality with endless possibilities in preclinical imaging.
The FIVE2 (ViewnVivo) from Optiscan Imaging is the latest and most competent model of LCE designed for effortless, real-time preclinical imaging in numerous animal models. This live webinar from two different imaging experts provided scientists and postdocs with the theoretical and practical knowledge about the FIVE2 (ViewnVivo) and its preclinical research applications. Former product manager of FIVE2 technology, Dr. Mohammedayaz Rangrez, gave a hands-on demonstration of the technology, imaging in different tissue types, Z sectioning and other key capabilities of the system. Dr. Howard Dobson from Invicro discussed some of its applications and image analysis illustrated with examples from normal and diseased rodents. This included images of the liver, kidneys and colon. Imaging of the colon was demonstrated using an intraabdominal approach in both rats and mice, as well as through a rectal approach in rats, making it ideally suited for longitudinal studies. In addition, the use of the FIVE2 (ViewnVivo) to image and quantify blood flow in small vessels was illustrated. Examples of quantitative image analysis approaches were included for each organ.
Topics to be discussed in this webinar included:
- Hardware functionality and software operation of the FIVE2 (ViewnVivo) – fluorescence in vivo endomicroscope
- How to use the FIVE2 (ViewnVivo) for imaging tissue architecture with cellular resolution and for optical sectioning
- Examples of normal and abnormal tissues in the abdominal cavity using a variety of fluorophores
- Overview of the approach to image analysis
- An understanding of applications of the FIVE2 (ViewnVivo) in preclinical research
About the Speaker (s)
Dr. Howard Dobson
Dr. Howard Dobson is a veterinary radiologist and is a Senior Director in the Scientific and Medical Services division at Invicro, a Boston based global imaging only contract research organisation. He has a broad background in all imaging modalities in all the major species from mice to monkeys. Prior to his work in contract research he was a faculty member at the Ontario Veterinary College, University of Guelph.
Dr. Mohammedayaz Rangrez
Dr. Mohammedayaz Rangrez is an imaging scientist with a Ph.D. in preclinical research on a bilateral ovariectomy model for osteoporosis. He has a broad background in microscopy, biochemistry, physiology and drug discovery. He was associated with the European Molecular Biology Laboratories, Heidelberg, Germany as a Technical Officer, developing e-learning courses on super-resolution microscopy. Previously, Dr. Rangrez was working as a Product Manager for in vivo microscopy at Scintica Instrumentation, with his expertise in application development and training. At present Dr. Rangrez is working as a research associate in Sunnybrook Hospital and University Health Network, Toronto, Canada.