WEBINAR: A Noninvasive Alternative to +dP/dtmax: Peak Aortic Blood Acceleration

Dr. Anil Reddy discusses how to use noninvasive blood flow velocity measurements to quantify cardiac contractility as a surrogate to +dP/dtmax.
Invasive measurement of left ventricular pressure is considered the gold standard for evaluating cardiac contractility and relaxation in preclinical research.  However, studying disease progression in the same subject over time is not possible, considering the acute and terminal nature of the protocol.  Fortunately, noninvasive imaging options do exist for measuring parameters such as ejection fraction, cardiac output, fractional shortening and cardiac dimensions, however the inherent variability of data from these systems does not provide enough confidence when running serial studies. During this webinar, sponsored by Indus Instruments, Dr. Anilkumar Reddy of the Baylor College of Medicine shows how peak acceleration of blood flow velocity in the ascending aorta can be used as a surrogate, noninvasive measurement to evaluate cardiac contractility. Importantly, this technique enables serial measurements in the same animal, leading to reduced animal-to-animal variability, fewer subjects and shorter data collection times. Specifically, Dr. Reddy presents preliminary findings from his validation studies in mice, where peak aortic acceleration and peak +dP/dt were compared. He highlights the utility of the Doppler method showing how measurements of mitral inflow and myocardial perfusion capacity through coronary flow reserve can be used to noninvasively assess diastolic function. Furthermore, he presents specific examples of noninvasive cardiac measurements and discuss how they scale in translational research from mice to mammals.
Key topics covered during this webinar included:
  • Evaluating cardiac contractility using peak aortic acceleration
  • Investigating cardiac relaxation using mitral peak early velocity to peak atrial velocity ratio
  • Interpreting myocardial perfusion capacity through coronary flow reserve at baseline and with disease or other conditions
  • How Doppler Flow Velocity measurements can be used in translational research from mice to mammals
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About the Speaker (s)

Anilkumar K. Reddy, Ph.D.

Associate Professor

Baylor College of Medicine, Texas

Dr. Reddy’s research interests include evaluation of cardiac and vascular mechanics in senescent, disease, transgenic, and surgical models of mice. Some of the rodent models he studies include atherosclerosis, dwarf, myocardial infarction/remodeling, pressure overload, hypertension, absent vascular tone, and absent steroid receptor coactivator-1, with the main goal being to translate what is learned in mice to humans for early detection and screening.

Using noninvasive methods, such as pulsed Doppler Flow Velocity measurements as well as imaging methods, animals are phenotyped as abnormalities develop and progress, and their cardiovascular system is monitored as it adapts and compensates for the deterioration of function or for missing or over-expressed proteins. The main goal is to translate what is learned in mice to humans for detection and screening of cardiovascular diseases at an early stage when potential therapies can be most effective at preventing disease progression.