(May 20, 2020) Webinar #1 – Modality Review: The Basics of Intravital Fluorescence Microscopy


This is the first of a 4-part series introducing Scintica’s newly formed relationship with IVIM Technology and their IntraVital Microscopy platform (IVM).

In this session, we introduced the fundamentals of fluorescence microscopy, review some example images, and focus on this technique’s intravital imaging applications. This webinar focused on formulating a basic understanding of the imaging modality to further understand the IVM system’s capabilities throughout the rest of the webinar series.

First, the fundamental principles of fluorescence imaging were explained, along with their advantages and challenges with applied in an in vivo setting. Next, we highlighted intravital microscopy’s advantages and its role in oncology research and other scientific areas. We also provided an overview of the most commonly used animal models for intravital imaging. Finally, we focused on the importance of acquiring quantitative imaging data and navigate around some pitfalls. Key examples from the research field were collected in this webinar.

After attending this webinar, attendees will have:

  • a basic understanding of the fundamentals of fluorescence microscopy,
  • an overview of intravital imaging advantages and applications,
  • an overview of the most commonly used intravital imaging animal models,
  • an understanding of what to pay attention to in order to acquire quantitative imaging data.]

Watch all IVIM Series here:

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About the Speaker (s)

Sara Rapic MSc, PhD

Manager, Imaging Division, Scintica

Sara Rapic has received extensive molecular imaging training in Europe and Canada since 2008. She holds an MSc in Molecular Imaging and a PhD in Medical Sciences from the University of Antwerp in Belgium. Her doctoral research focused on the evaluation of novel radiotracers as biomarkers of treatment response in a colorectal cancer model using nuclear imaging technologies. During her postdoctoral fellowship at the Princess Margaret Cancer Centre in Toronto, Canada, she studied the complex tumor microenvironment of pancreatic cancer using intravital microscopy. In her present role as Scientific Product Manager at Scintica Instrumentation, Sara supports our customers in understanding the products offered and how these instruments help to meet their research needs.