Frequently Asked Questions : Fluorescence & Bioluminescence Imaging System
This frequently asked questions document has been designed to help answer some of the most common questions related to preclinical optical imaging using the Newton 7.0 system.
1. What imaging modalities are supported by the
Newton 7.0
The Newton 7.0 system is designed for preclinical optical imaging, supporting both bioluminescence imaging (BLI) and fluorescence imaging (FLI). It accommodates in vivo, ex vivo, and in vitro studies across small animal models, tissue samples, and cell cultures. It also includes a 3D bioluminescence topography module, which reconstructs optical signals into 3D space and overlays them within a topographic model of the mouse to provide more accurate localization of molecular signals within the body.
2. What kind of camera and sensitivity does the Newton use?
The Newton 7.0 is equipped with a high-resolution, deep-cooled CCD camera capable of detecting low-light emissions with exceptional sensitivity. Cooling enhances long-exposure acquisitions by reducing background noise. Detect even the faintest signals with the Newton 7.0.
3. How does the system handle tissue auto-fluorescence?
Auto-fluorescence can be reduced using background subtraction. We recommend using NIR fluorophores, which are less prone to interference from tissue autofluorescence and provide better signal-to-noise ratios.
4. How many animals can be imaged at once?Â
The FT-100 and BT-100 accommodate 3 mice or 1 rat (fixed 12×12 cm FOV). The FT-500 and BT-500 accommodate 5 mice or 3 rats simultaneously (6×6 cm up to 20×20 cm FOV). A 10-mouse platform is available.
5. Is the system user-friendly?
Yes. The system features an intuitive user interface, automated acquisition
protocols, and real-time image previews. Image acquisition and post-processing
are handled via a dedicated Vilber software suite, which includes tools for
quantification, 3D reconstruction, and signal comparison. All software is licensefree with unlimited seats.
6. Can I use my existing anesthesia system?
Yes. The Newton 7.0 is compatible with most standard small animal anesthesia systems. Scintica also offers integrated anesthesia solutions, which fit seamlessly into the imaging platform for user convenience and consistent animal sedation.
7. Is the Newton 7.0 suitable for labs new to optical imaging?
Absolutely. The system is ideal for both experienced imaging cores and first-time users. Its ease of use, comprehensive support, and scalable configuration make it a future-proof investment for a range of applications including oncology, inflammation, infection models, and gene expression studies.
8. Is the Newton 7.0 capable of imaging in the NIR-II window?
Scintica is pleased to offer the Newton FT-900, which is an in vivo optical imaging system dedicated to SWIR (NIR-I, NIR-II) fluorescence and bioluminescence imaging. Click here to learn more.
